UN SDG
Horticultural Studies (HortiS)
2008, Vol 25, Num, 1 (Pages: 59-69)
COMPARISONS OF DNA ISOLATION METHODS FOR SOME FRUIT SPECIES
2 Çukurova Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Balcalı / ADANA
3 Mustafa Kemal Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü / HATAY Viewed : 1533 - Downloaded : 1986 Virtually all molecular biology studied requires high quality DNA isolated from plants of interest. Therefore, it is important to isolate pure and high quality DNA in a short time. In this study, DNA sampled from hazelnut, avocado, persimmon, mandarin and orange were isolated using MiniPrep DNA isolation methods and its modified versions. These treatments were prepared adding 1) CTAB, 2) CTAB and PVP (CTAB+PVP), 3) CTAB and SDS (CTAB+SDS) 4) SDS to extraction buffer. The DNA samples isolated using these methods were compared for their concentrations and purity. In general, all method yielded high DNA concentrations; the differences among the fruit species and the isolation methods were found to be statistically significant. The highest DNA concentrations were recovered from hazelnut and orange while CTAB+SDS yielded significantly lower DNA concentration when compared to the other methods. Keywords : DNA isolation, MiniPrep, CTAB, PVP, SDS
