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¹ Akdeniz Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü, Antalya, Türkiye Viewed : 1739 - Downloaded : 925 Micropropagation is a plant tissue culture technique that can yield a large number of genetically identical plantlets. This technique has been commercially used in horticulture, field crops, forestry and landscape architecture. Controlling in vitro environment is important for producing high quality plantlets with efficient use of energy. In this article, we evaluate environmental control factors currently used in micropropagation such as aerial environmental factors including light, carbon dioxide concentration, temperature, relative air humidity and oxygen, the effects of culture medium physical and chemical environmental factors including sugar concentration, concentrations of ion components and pH of the culture medium together with the effects of biological factors such as explant size, explant density and pathogens on the growth and photosynthesis of plantlets in vitro are discussed. Keywords : Photoautotrophic micropropagation, CO₂, Sugar, Light