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¹ Isparta University of Applied Sciences, Faculty of Agriculture, Horticulture Department, 32200, Isparta, Turkey DOI : 10.16882/HortiS.1069414 Viewed : 1454 - Downloaded : 1255 Fusarium oxysporum f. sp. lycopersici, Verticillium spp., and nematodes, as well as virus diseases that negatively affect production with limited chemical control cause significant losses in greenhouse tomato cultivation. The practical and effective side of controlling diseases is genetic control by breeding. Using a genomic approach for plant breeding is a more sustainable and effective way to control disease and pests. The development of the resistant line is improved by conventional breeding methods that can be conducted over a long period. However, molecular markers make these processes considerably shorter with identifying resistant individuals. In this study, 14 wild and 188 cultivated tomato genotypes have been tested against Fusarium oxysporum f. sp. radicis-lycopersici (FORL), Meloidogyne spp. (root-knot nematodes), Tomato spotted wilt virus (TSWV), Tomato mosaic virus (ToMV), Tomato yellow leaf curl virus (TYLCV) and Verticillum dahliae Kleb. (Ve) diseases and pests with using MAS (Marker- Assisted Selection) technique. According to these results, it has been determined that the selected markers can be used effectively in breeding studies to determine the diseases mentioned above. Keywords : Disease resistant MAS Molecular markers Tomato