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¹ Ege University, Faculty of Science, Department of Biology, 35040, İzmir, Türkiye
² İzmir Demokrasi University, Vocational High School, 35140, İzmir, Türkiye DOI : 10.16882/HortiS.1200592 Viewed : 1138 - Downloaded : 453 Campanula phitosiana Yıldırım & Şentürk, is a local endemic to the Aydın Mountain range, which distribute across in Western Anatolia (both İzmir and Aydın). This species belongs to the Mediterranean basin floristic region. According to the International Union for Conservation of Nature`s criteria, C. phitosiana is classified as `Critically Endangered` (CR). The aim of the study is to develop in vitro regeneration protocol for critically endangered endemic Campanula phitosiana. To investigate the efficient medium and plant growth regulator combinations for callus initiation and shoot proliferation, petiole and leaves were used as explant and explants cultivated on MS medium including NAA (1-Naphthylacetic acid) (0.3 mg L^-1), TDZ (Thidiazuron) (0.5, 1.0, 2.0, and 3.0 mg L^-1), BA (6-Benzylaminopurine) (0.5, 1.0, 2.0, and 3.0 mg L^-1) and Gibberellic Acid (GA3) (0.5, 1.0, 2.0, and 3.0 mg L^-1). Explants subcultured 3 times. Experiments were conducted according to completely randomized design repeated with 5 replicates and each replicates including 5 explants. As a result of the experiment, callus initiation and shoot proliferation were investigated. Efficient callus initiation was observed petiole explants as 100% rate. Shoot proliferation was observed on MS medium including 0.3 mg L^-1 NAA + 2.0 mg L^-1 GA₃. Keywords : Campanula Endemic Plant growth regulator Regeneration Tissue culture