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¹ Batı Akdeniz Tarımsal Araştırma Enstitüsü, Antalya DOI : 10.16882/derim.2018.325765 Viewed : 2082 - Downloaded : 2013 Pepper is widely grown vegetable crops after tomato and watermelon in the world. One of the most important diseases on pepper production is caused by Tomato spotted wilt virus (TSWV). The best way for the control of this disease is to use resistant varieties. This study aimed to improve TSWV resistant lines to use as a parent in long type pepper breeding studies. Three genotypes resistant to TSWV were crossed with the susceptible Serademre 8 used to be commercial. Pedigree method was applied as the breeding method. Three populations were self-pollinated for five generations. The progenies for each of populations were tested against TSWV. Mechanical inoculations were performed in each generation and the selected resistant plants were grown for selfpollination to achieve next generation. Molecular tests were applied to the plants in stages F₁ and F₅. Virulent strain isolated from infected pepper plants was used as a disease material in the mechanical inoculations. CAPS marker (SCAC₅₆₈) linked to the TSWV resistance gene (Tsw) was used in the molecular test for verification of mechanical test. In this study, 10 lines resistant to TSWV with good features were developed. Some of the obtained TSWV resistant lines have been transferred to private sector seed companies for use in pepper breeding programs. Keywords : Pepper; TSWV; Mechanical inoculation; Molecular marker; CAPS